Klebsiella 2024

An introductory course on Klebsiella pneumoniae bioinfomatics

Programme

The course is a two week programme of in-person instruction combining theoretical and practical instruction with an opportunity for hands-on practice of the skills presented.

Week 1
Day 1 (Mon) Day 2 (Tue) Day 3 (Wed) Day 4 (Thu) Day 5 (Fri)
09h00 Welcome: What do we hope to achieve?

Speed Dating Introductions
Pathogenwatch wrap-up

Talk: Introduction to data visualisation in Microreact
Applications of Pathogen Genomics

Outbreak simulation workshop
Policy and datasharing

Possible Talks (1 or 2 invited speakers)
Kaptive hands on practical: review and discussion

Klebsiella virulence typing part II
11h00 Tea Break (15 min)
11h15 Recorded talk: Practical aspects of Pathogen Genomics

Pathogenwatch introduction
Pathogenwatch practical
Microreact practical Outbreak simulation workshop An introdcution to Klebsiella genomics and strain typing

Klebsiella virulence typing part I
Klebsiella AMR & plasmid typing

Kleborate hands on practical
13h00 Lunch Break
14h00 Pathogenwatch practical Microreact Practical & wrap-up

Recorded talk: Title TBC (Natacha Couto)
Outbreak simulation workshop

Talk: Genomic surveillance of AMR using amr.watch (Julio Diaz-Caballero)
Kaptive hands on practical Kleborate hands on practical
15h15 Tea Break (15 min)
15h30 - 16:30 Talk: Genomic surveillance of K. pneumoniae in Europe Data sharing workflow mapping Data sharing workflow mapping Data sharing workflow mapping Kleborate hands on practical: review & discussion

Data sharing workflow mapping

Week 2
Day 6 (Mon) Day 7 (Tue) Day 8 (Wed) Day 9 (Thu) Day 10 (Fri)
09h00 Introduction to CLI: hands-on

Theory: File formats, long vs short reads, raw reads QC
Hands on: variant calling Cholera case study Project presentations
11h00 Tea Break (15 min)
11h15 Theory and Hands on; Conda + tool installation Hands on: phylogenetics PRACTICAL SESSION: (Trainees to work on projects) Project presentations
13h00 Lunch Break
14h00 Theory and Hands on QC & alignment Hands on: phylodynamics PRACTICAL SESSION: (Trainees to work on projects) Project presentations
15h15 Tea Break (15 min)
15h30 - 16:30 Theory and Hands on QC & alignment Hands on: phylodynamics PRACTICAL SESSION: (Trainees to work on projects) Project presentations

Partners

This course is a joint collaboration between SANBI (the South African National Bioinformatics Institute) at the University of the Western Cape (UWC), Africa CDC (the Africa Centres for Disease Control and Prevention), part of the African Union (AU), African Society for Laboratory Medicine (ASLM), London School of Hygiene and Tropical Medicine (LSHTM) and Big Data Institute at the University of Oxford